The Mariappan Lab
Publications
2023
30. Sun S, Li X, Mariappan M. Signal sequences encode information for protein folding in the endoplasmic reticulum.
J Cell Biol. 2023 Jan 2;222(1):e202203070. doi: 10.1083/jcb.202203070.
Highlighted by Jon Atherton: Decoding the cell signals between young proteins and their ‘chaperones’
https://westcampus.yale.edu/news/decoding-cell-signals-between-young-proteins-and-their-chaperones
2022
29. Heo P, Culver JA, Miao J, Pincet F, Mariappan M. The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ER. Cell Reports. 2022 Dec 28;42(1):111921. doi: 10.1016/j.celrep.2022.111921.
28. Culver JA, Li X, Jordan M, Mariappan M. A second chance for protein targeting/folding: Ubiquitination and deubiquitination of nascent proteins. Bioessays. 2022 Mar 31:e2200014.
27. Giska F, Mariappan M, Bhattacharyya M, Gupta K. Deciphering the molecular organization of GET pathway chaperones through native mass spectrometry. Biophys J. 2022 Feb 19:S0006-3495(22)00150-3.
2021
26. Culver JA, Mariappan M. Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
Journal of Cell Biology. 2021 May 3;220(5):e202004086. doi: 10.1083/jcb.202004086. PMID: 33792613;
PMCID: PMC8020466.
- Highlighted in Journal of Cell Biology Spotlight: A TRCky TA protein delivery
service snubs the UPS by McQuown AJ, Reif D, Denic V.
25. Jordan M and Mariappan M. "Tail-Anchored Protein Insertion Under ER Stress Conditions: Calcium is Key,"
The Yale Undergraduate Research Journal. 2021 Vol. 2: Iss. 1, Article 32.
2020
24. Li X, Sun S, Appathurai S, Sundaram A, Plumb R, Mariappan M. A Molecular Mechanism for Turning Off IRE1α Signaling during Endoplasmic Reticulum Stress. Cell Reports 2020 Dec 29;33(13):108563. doi: 10.1016/j.celrep.2020.108563. PMID: 33378667; PMCID: PMC7809255.
Highlighted in Yale Scientific Magazine: Shutting Down the IRE1α Complex
by Ryan Bose-Roy April 27, 2021
23. Culver JA and Mariappan M. Membrane Protein Biogenesis: PAT Complex Pats Membrane Proteins into Shape.
Current Biology. 2020 Nov 16;30(22):R1387-R1389. doi: 10.1016/j.cub.2020.09.072. PMID:
33202243.
22. Sun S and Mariappan M. C-terminal tail length guides insertion and assembly of membrane proteins. J Biol Chem.
2020 Nov 13;295(46):15498-15510. doi: 10.1074/jbc.RA120.012992. PMID: 32878985; PMCID: PMC7667985.
The top 50 most viewed papers in JBC in September and October of 2020.
21. Sun S and Mariappan M. Lonely ER Membrane Proteins Travel to the Nucleus to Rest in Peace by the Asi Complex.
Molecular Cell. 2020 Jan 2;77(1):1-2.
2019
20. Albert, A., Adua, A., Cai, W., Cline, G., Liu, Z., Zhao, M., Arnal-Estapé, A., Cao, P., Mariappan, M., and Nguyen., D.
Adaptive protein translation by the integrated stress response maintains the proliferative and migratory capacity of
lung adenocarcinoma cells. Mol Cancer Res. 2019 Dec;17(12):2343-2355.
2018
19. Sundaram A, Appathurai S, Plumb R, Mariappan M. Dynamic changes in complexes of IRE1α, PERK, and ATF6α
during endoplasmic reticulum stress. Mol Biol Cell. 2018 Jun 1;29(11):1376-1388.
2017
18. *Sundaram A, *Plumb R, Appathurai S, Mariappan M. The Sec61 translocon limits IRE1α signaling during the
unfolded protein response. eLIFE. 2017, 6. pii: e27187. doi: 10.7554/eLife.27187.
2015
17. Peng J, Alam S, Radhakrishnan K, Mariappan M, Rudolph MG, May C, Dierks T, von Figura K, Schmidt B. Eukaryotic
formylglycine-generating enzyme catalyses a monooxygenase type of reaction. FEBS J. 2015, 282(17):3262-74.
16. *Plumb R, *Zhang ZR, Appathurai S, Mariappan M. A functional link between the co-translational protein
translocation pathway and the UPR. eLIFE. 2015, 4. doi: 10.7554/eLife.07426.
15. Ennemann EC, Radhakrishnan K, Mariappan M, Wachs M, Pringle TH, Schmidt B, Dierks T. Proprotein convertases
process and thereby inactivate formylglycine-generating enzyme. J Biol Chem. 2013, 288(8):5828-39.
Before coming to Yale
14. Sherrill J, Mariappan M, Dominik P, Hegde RS, Keenan RJ. A conserved archaeal pathway for tail-anchored membrane
protein insertion. Traffic. 2011, 12(9):1119-23.
13. *Mariappan M, *Mateja A, Dobosz M, Bove E, Hegde RS, Keenan RJ. The mechanism of membrane-associated steps in
tail-anchored protein insertion. Nature. 2011, 477(7362):61-6.
12. Hessa T, Sharma A, Mariappan M, Eshleman HD, Gutierrez E, Hegde RS. Protein targeting and degradation are
coupled for elimination of mislocalized proteins. Nature. 2011, 475(7356):394-7.
- High lighted in Current Biology: BAGing Up the Trash
- Nominated to F1000
11. Mariappan M, Li X, Stefanovic S, Sharma A, Mateja A, Keenan RJ, Hegde RS. A ribosome-associating factor chaperones
tail-anchored membrane proteins. Nature. 2010, 466(7310):1120-4.
- Nominated to F1000
10. Sharma A, Mariappan M, Appathurai S, Hegde RS. In vitro dissection of protein translocation into the mammalian
endoplasmic reticulum. Methods Mol Biol. 2010, 619:339-63.
9. Mateja A, Szlachcic A, Downing ME, Dobosz M, Mariappan M, Hegde RS, Keenan RJ. The structural basis of tail-
anchored membrane protein recognition by Get3. Nature. 2009, 461(7262):361-6.
- High lighted in Nature StructuralBiology: Get3 into the groove
- Nominated to F1000
8. Mariappan M, Gande SL, Radhakrishnan K, Schmidt B, Dierks T, von Figura K. The non-catalytic N-terminal extension
of formylglycine-generating enzyme is required for its biological activity and retention in the endoplasmic reticulum. J
Biol Chem. 2008, 283(17):11556-64.
7. *Mariappan M, *Radhakrishnan K, Dierks T, Schmidt B, von Figura K. ERp44 mediates a thiol-independent retention
of formylglycine-generating enzyme in the endoplasmic reticulum. J Biol Chem. 283(10):6375-83.
6. Gande SL, Mariappan M, Schmidt B, Pringle TH, von Figura K, Dierks T. Paralog of the formylglycine-generating
enzyme--retention in the endoplasmic reticulum by canonical and noncanonical signals. FEBS J. 2008,
275(6):1118-30.
5. Dierks T, Dickmanns A, Preusser-Kunze A, Schmidt B, Mariappan M, von Figura K, Ficner R, Rudolph MG. Molecular
basis for multiple sulfatase deficiency and mechanism for formylglycine generation of the human formylglycine-
generating enzyme. Cell. 2005, 121(4):541-52.
4. Mariappan M, Preusser-Kunze A, Balleininger M, Eiselt N, Schmidt B, Gande SL, Wenzel D, Dierks T, von Figura K.
Expression, localization, structural, and functional characterization of pFGE, the paralog of the Calpha-formylglycine-
generating enzyme. J Biol Chem. 2005, 280(15):15173-9.
3. Dickmanns A, Schmidt B, Rudolph MG, Mariappan M, Dierks T, von Figura K, Ficner R. Crystal structure of human
pFGE, the paralog of the Calpha-formylglycine-generating enzyme. J Biol Chem. 2005, 280(15):15180-7. 15687489.
2. *Preusser-Kunze A, *Mariappan M, Schmidt B, Gande SL, Mutenda K, Wenzel D, von Figura K, Dierks T. Molecular
characterization of the human Calpha-formylglycine-generating enzyme. J Biol Chem. 2005, 280(15):14900-10.
1. Dierks T, Schmidt B, Borissenko LV, Peng J, Preusser A, Mariappan M, von Figura K. Multiple sulfatase deficiency is
caused by mutations in the gene encoding the human C(alpha)-formylglycine generating enzyme. Cell. 2003 May
16;113(4):435-44.
- Preview by Jacques U Baenziger: A major step on the road to understanding a unique
posttranslational modification and its role in a genetic disease Cell. 2003, 16;113 (4):421-2.