The Mariappan Lab
Publications
2023
33. Nascent Chain Ubiquitination is Uncoupled from Degradation to Enable Protein Maturation
Li X. and Mariappan M. bioRxiv 2023.10.09.561585; doi: https://doi.org/10.1101/2023.10.09.561585.
32. Thoduvayil S, Weerakkody JS, Sundaram RVK, Topper M, Bera M, Coleman J, Li X, Mariappan M, Ramakrishnan S.
Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving
Insulin Therapy. Cell Calcium. 2023 Jun 3;113:102766. PMID: 37295201.
31. Subramanian K, Paul S, Libby A, Patterson J, Arterbery A, Knight J, Castaldi C, Wang G, Avitzur Y, Martinez M,
Lobritto S, Deng Y, Geliang G, Kroemer A, Fishbein T, Mason A, Dominguez-Villar M, Mariappan M, Ekong UD.
HERV1-env Induces Unfolded Protein Response Activation in Autoimmune Liver Disease: A Potential Mechanism for Regulatory T Cell Dysfunction. J Immunology. 2023 Feb 1:ji2100186. PMID: 36722941.
30. Sun S, Li X, Mariappan M. Signal sequences encode information for protein folding in the endoplasmic reticulum.
J Cell Biol. 2023 Jan 2;222(1):e202203070. doi: 10.1083/jcb.202203070.
Highlighted by Jon Atherton: Decoding the cell signals between young proteins and their ‘chaperones’
https://westcampus.yale.edu/news/decoding-cell-signals-between-young-proteins-and-their-chaperones
2022
29. Heo P, Culver JA, Miao J, Pincet F, Mariappan M. The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ER. Cell Reports. 2022 Dec 28;42(1):111921. doi: 10.1016/j.celrep.2022.111921.
28. Culver JA, Li X, Jordan M, Mariappan M. A second chance for protein targeting/folding: Ubiquitination and deubiquitination of nascent proteins. Bioessays. 2022 Mar 31:e2200014.
27. Giska F, Mariappan M, Bhattacharyya M, Gupta K. Deciphering the molecular organization of GET pathway chaperones through native mass spectrometry. Biophys J. 2022 Feb 19:S0006-3495(22)00150-3.
2021
26. Culver JA, Mariappan M. Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
Journal of Cell Biology. 2021 May 3;220(5):e202004086. doi: 10.1083/jcb.202004086. PMID: 33792613;
PMCID: PMC8020466.
- Highlighted in Journal of Cell Biology Spotlight: A TRCky TA protein delivery
service snubs the UPS by McQuown AJ, Reif D, Denic V.
25. Jordan M and Mariappan M. "Tail-Anchored Protein Insertion Under ER Stress Conditions: Calcium is Key,"
The Yale Undergraduate Research Journal. 2021 Vol. 2: Iss. 1, Article 32.
2020
24. Li X, Sun S, Appathurai S, Sundaram A, Plumb R, Mariappan M. A Molecular Mechanism for Turning Off IRE1α Signaling during Endoplasmic Reticulum Stress. Cell Reports 2020 Dec 29;33(13):108563. doi: 10.1016/j.celrep.2020.108563. PMID: 33378667; PMCID: PMC7809255.
Highlighted in Yale Scientific Magazine: Shutting Down the IRE1α Complex
by Ryan Bose-Roy April 27, 2021
23. Culver JA and Mariappan M. Membrane Protein Biogenesis: PAT Complex Pats Membrane Proteins into Shape.
Current Biology. 2020 Nov 16;30(22):R1387-R1389. doi: 10.1016/j.cub.2020.09.072. PMID:
33202243.
22. Sun S and Mariappan M. C-terminal tail length guides insertion and assembly of membrane proteins. J Biol Chem.
2020 Nov 13;295(46):15498-15510. doi: 10.1074/jbc.RA120.012992. PMID: 32878985; PMCID: PMC7667985.
The top 50 most viewed papers in JBC in September and October of 2020.
21. Sun S and Mariappan M. Lonely ER Membrane Proteins Travel to the Nucleus to Rest in Peace by the Asi Complex.
Molecular Cell. 2020 Jan 2;77(1):1-2.
2019
20. Albert, A., Adua, A., Cai, W., Cline, G., Liu, Z., Zhao, M., Arnal-Estapé, A., Cao, P., Mariappan, M., and Nguyen., D.
Adaptive protein translation by the integrated stress response maintains the proliferative and migratory capacity of
lung adenocarcinoma cells. Mol Cancer Res. 2019 Dec;17(12):2343-2355.
2018
19. Sundaram A, Appathurai S, Plumb R, Mariappan M. Dynamic changes in complexes of IRE1α, PERK, and ATF6α
during endoplasmic reticulum stress. Mol Biol Cell. 2018 Jun 1;29(11):1376-1388.
2017
18. *Sundaram A, *Plumb R, Appathurai S, Mariappan M. The Sec61 translocon limits IRE1α signaling during the
unfolded protein response. eLIFE. 2017, 6. pii: e27187. doi: 10.7554/eLife.27187.
2015
17. Peng J, Alam S, Radhakrishnan K, Mariappan M, Rudolph MG, May C, Dierks T, von Figura K, Schmidt B. Eukaryotic
formylglycine-generating enzyme catalyses a monooxygenase type of reaction. FEBS J. 2015, 282(17):3262-74.
16. *Plumb R, *Zhang ZR, Appathurai S, Mariappan M. A functional link between the co-translational protein
translocation pathway and the UPR. eLIFE. 2015, 4. doi: 10.7554/eLife.07426.
15. Ennemann EC, Radhakrishnan K, Mariappan M, Wachs M, Pringle TH, Schmidt B, Dierks T. Proprotein convertases
process and thereby inactivate formylglycine-generating enzyme. J Biol Chem. 2013, 288(8):5828-39.
Before coming to Yale
14. Sherrill J, Mariappan M, Dominik P, Hegde RS, Keenan RJ. A conserved archaeal pathway for tail-anchored membrane
protein insertion. Traffic. 2011, 12(9):1119-23.
13. *Mariappan M, *Mateja A, Dobosz M, Bove E, Hegde RS, Keenan RJ. The mechanism of membrane-associated steps in
tail-anchored protein insertion. Nature. 2011, 477(7362):61-6.
12. Hessa T, Sharma A, Mariappan M, Eshleman HD, Gutierrez E, Hegde RS. Protein targeting and degradation are
coupled for elimination of mislocalized proteins. Nature. 2011, 475(7356):394-7.
- High lighted in Current Biology: BAGing Up the Trash
- Nominated to F1000
11. Mariappan M, Li X, Stefanovic S, Sharma A, Mateja A, Keenan RJ, Hegde RS. A ribosome-associating factor chaperones
tail-anchored membrane proteins. Nature. 2010, 466(7310):1120-4.
- Nominated to F1000
10. Sharma A, Mariappan M, Appathurai S, Hegde RS. In vitro dissection of protein translocation into the mammalian
endoplasmic reticulum. Methods Mol Biol. 2010, 619:339-63.
9. Mateja A, Szlachcic A, Downing ME, Dobosz M, Mariappan M, Hegde RS, Keenan RJ. The structural basis of tail-
anchored membrane protein recognition by Get3. Nature. 2009, 461(7262):361-6.
- High lighted in Nature StructuralBiology: Get3 into the groove
- Nominated to F1000
8. Mariappan M, Gande SL, Radhakrishnan K, Schmidt B, Dierks T, von Figura K. The non-catalytic N-terminal extension
of formylglycine-generating enzyme is required for its biological activity and retention in the endoplasmic reticulum. J
Biol Chem. 2008, 283(17):11556-64.
7. *Mariappan M, *Radhakrishnan K, Dierks T, Schmidt B, von Figura K. ERp44 mediates a thiol-independent retention
of formylglycine-generating enzyme in the endoplasmic reticulum. J Biol Chem. 283(10):6375-83.
6. Gande SL, Mariappan M, Schmidt B, Pringle TH, von Figura K, Dierks T. Paralog of the formylglycine-generating
enzyme--retention in the endoplasmic reticulum by canonical and noncanonical signals. FEBS J. 2008,
275(6):1118-30.
5. Dierks T, Dickmanns A, Preusser-Kunze A, Schmidt B, Mariappan M, von Figura K, Ficner R, Rudolph MG. Molecular
basis for multiple sulfatase deficiency and mechanism for formylglycine generation of the human formylglycine-
generating enzyme. Cell. 2005, 121(4):541-52.
4. Mariappan M, Preusser-Kunze A, Balleininger M, Eiselt N, Schmidt B, Gande SL, Wenzel D, Dierks T, von Figura K.
Expression, localization, structural, and functional characterization of pFGE, the paralog of the Calpha-formylglycine-
generating enzyme. J Biol Chem. 2005, 280(15):15173-9.
3. Dickmanns A, Schmidt B, Rudolph MG, Mariappan M, Dierks T, von Figura K, Ficner R. Crystal structure of human
pFGE, the paralog of the Calpha-formylglycine-generating enzyme. J Biol Chem. 2005, 280(15):15180-7. 15687489.
2. *Preusser-Kunze A, *Mariappan M, Schmidt B, Gande SL, Mutenda K, Wenzel D, von Figura K, Dierks T. Molecular
characterization of the human Calpha-formylglycine-generating enzyme. J Biol Chem. 2005, 280(15):14900-10.
1. Dierks T, Schmidt B, Borissenko LV, Peng J, Preusser A, Mariappan M, von Figura K. Multiple sulfatase deficiency is
caused by mutations in the gene encoding the human C(alpha)-formylglycine generating enzyme. Cell. 2003 May
16;113(4):435-44.
- Preview by Jacques U Baenziger: A major step on the road to understanding a unique
posttranslational modification and its role in a genetic disease Cell. 2003, 16;113 (4):421-2.